Hotspot

trnK-UUU-rps16

LSC · 4474-6253 · 1780 bp

Back to Myrospermum

Pi 0.135
MarkerSeek score 85.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1780
variable_sites
220
pi
0.135
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGCCGAGTACTCTACCATTGA AAGGCGGGTGTTTTTATGGA 47.6 45.0 58.0 57.7 1811-1866 bp 1.0 81.5
trnK-UUU-rps16_p2 AGCCGAGTACTCTACCATTGA AGGCGGGTGTTTTTATGGAA 47.6 45.0 58.0 57.7 1810-1865 bp 1.0 81.5
trnK-UUU-rps16_p3 AGCCGAGTACTCTACCATTGA GGCGGGTGTTTTTATGGAAC 47.6 50.0 58.0 57.6 1809-1864 bp 1.0 81.0
trnK-UUU-rps16_p4 GCCGAGTACTCTACCATTGAG AAGGCGGGTGTTTTTATGGA 52.4 45.0 57.6 57.7 1810-1865 bp 1.0 78.8
trnK-UUU-rps16_p5 GCCGAGTACTCTACCATTGAG AGGCGGGTGTTTTTATGGAA 52.4 45.0 57.6 57.7 1809-1864 bp 1.0 78.8