Hotspot

rpl32-trnL-UAG

SSC · 122181-123841 · 1661 bp

Back to Myrocarpus

Pi 0.0206

MarkerSeek score 74.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1661
variable_sites: 32
pi: 0.0206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCAATCTCTTTCTACGGGCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1764-1931 bp	1.0	84.2
rpl32-trnL-UAG_p2	AGCGCAATCTCTTTCTACGG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.1	57.9	1767-1934 bp	1.0	83.8
rpl32-trnL-UAG_p3	GCAATCTCTTTCTACGGGCA	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	1757-1924 bp	1.0	83.7
rpl32-trnL-UAG_p4	GCAATCTCTTTCTACGGGCA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.0	57.8	1763-1930 bp	1.0	83.6
rpl32-trnL-UAG_p5	GCAATCTCTTTCTACGGGCA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	1762-1929 bp	1.0	83.6