Hotspot

trnN-GUU-ndhF

IRb · 118196-118683 · 488 bp

Back to Myrocarpus

Pi 0.0331
MarkerSeek score 68.00
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
IRb
length_bp
488
variable_sites
16
pi
0.0331
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 ACGACCAGTCAGTTAACAGC GGGGGTGGACGAATTTCTTC 50.0 55.0 57.8 58.5 621-674 bp 1.0 77.0
trnN-GUU-ndhF_p2 AGGATTCGAACCTACGACCA GGGGGTGGACGAATTTCTTC 50.0 55.0 58.2 58.5 634-687 bp 1.0 76.9
trnN-GUU-ndhF_p3 GGATTCGAACCTACGACCAG GGGGGTGGACGAATTTCTTC 55.0 55.0 57.8 58.5 633-686 bp 1.0 76.5
trnN-GUU-ndhF_p4 CCCCAAGTAGGATTCGAACC GGGGGTGGACGAATTTCTTC 55.0 55.0 57.7 58.5 642-695 bp 1.0 75.5
trnN-GUU-ndhF_p5 TTCGAACCTACGACCAGTCA GGGGGTGGACGAATTTCTTC 50.0 55.0 58.4 58.5 630-683 bp 1.0 75.0