Hotspot

rbcL-accD

LSC · 62665-64227 · 1563 bp

Back to Myrocarpus

Pi 0.0296

MarkerSeek score 66.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1563
variable_sites: 44
pi: 0.0296
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AATGAGGGACGTGATCTTGC	AATTCCGGATGTTCCTGACG	50.0	50.0	58.0	58.0	1921-1968 bp	1.0	84.5
rbcL-accD_p2	TGGAGATCATGTTCACGCTG	AATTCCGGATGTTCCTGACG	50.0	50.0	58.0	58.0	2249-2296 bp	1.0	84.0
rbcL-accD_p3	GAGATCATGTTCACGCTGGT	AATTCCGGATGTTCCTGACG	50.0	50.0	58.0	58.0	2247-2294 bp	1.0	84.0
rbcL-accD_p4	GTGGAGATCATGTTCACGCT	AATTCCGGATGTTCCTGACG	50.0	50.0	58.0	58.0	2250-2297 bp	1.0	84.0
rbcL-accD_p5	ATCATGTTCACGCTGGTACC	AATTCCGGATGTTCCTGACG	50.0	50.0	58.0	58.0	2244-2291 bp	1.0	83.9