Hotspot

trnV-UAC-trnM-CAU

LSC · 58008-58187 · 180 bp

Back to Myrocarpus

Pi 0.0391

MarkerSeek score 75.50

Primer available yes

Label trnV-UAC-trnM-CAU

Feature Metadata

label_name: trnV-UAC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 7
pi: 0.0391
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-trnM-CAU_p1	CGTGTAAACGAGGTGCTCTA	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.4	58.4	263-264 bp	1.0	74.1
trnV-UAC-trnM-CAU_p2	CGTGTAAACGAGGTGCTCTA	CCGCCGTATGAAAGCAATAC	50.0	50.0	57.4	57.3	262-263 bp	1.0	72.1
trnV-UAC-trnM-CAU_p3	CGTGTAAACGAGGTGCTCTA	TTTGAACCAATGACTCCCGC	50.0	50.0	57.4	58.8	278-279 bp	1.0	71.3
trnV-UAC-trnM-CAU_p4	CGTGTAAACGAGGTGCTCTA	GACTCCCGCCGTATGAAAG	50.0	57.9	57.4	58.0	267-268 bp	1.0	69.2
trnV-UAC-trnM-CAU_p5	CGTGTAAACGAGGTGCTCTA	TTGAACCAATGACTCCCGC	50.0	52.6	57.4	58.1	277-278 bp	1.0	68.8