Hotspot

trnG-GCC-trnfM-CAU

LSC · 42174-42388 · 215 bp

Back to Myrocarpus

Pi 0.0279

MarkerSeek score 68.50

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 215
variable_sites: 6
pi: 0.0279
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	314-419 bp	1.0	78.2
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	313-418 bp	1.0	78.2
trnG-GCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	ATTCTTCTCTTTACCGCGGG	50.0	50.0	57.8	57.7	347-452 bp	1.0	77.4
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	AGGTCATGGGTTCAAATCCC	50.0	50.0	57.8	57.5	289-394 bp	1.0	75.8
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	CCTTGAGGTCATGGGTTCAA	50.0	50.0	57.8	57.4	294-399 bp	1.0	75.3