Hotspot

trnC-GCA-petN

LSC · 32318-32952 · 635 bp

Back to Myrocarpus

Pi 0.0192

MarkerSeek score 71.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 635
variable_sites: 12
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	57.9	58.0	754-761 bp	1.0	81.7
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	698-704 bp	1.0	81.7
trnC-GCA-petN_p3	TTTTGTATCGTTTTGGCGGC	CCATACGACGAGTGAAAGGG	45.0	55.0	57.9	58.1	792-799 bp	1.0	81.1
trnC-GCA-petN_p4	TTTTGTATCGTTTTGGCGGC	CCCATACGACGAGTGAAAGG	45.0	55.0	57.9	58.1	793-800 bp	1.0	81.1
trnC-GCA-petN_p5	TTTTGTATCGTTTTGGCGGC	CCCCATACGACGAGTGAAAG	45.0	55.0	57.9	58.1	794-801 bp	1.0	81.1