Hotspot

trnQ-UUG-psbK

LSC · 8108-9500 · 1393 bp

Back to Myrocarpus

Pi 0.026

MarkerSeek score 67.10

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 1393
variable_sites: 32
pi: 0.026
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	CTTCGAATAGCGGGACCAAA	ACTTGAATAAAGGGCAGAGTGA	50.0	40.9	58.0	57.4	1501-1590 bp	1.0	64.4
trnQ-UUG-psbK_p2	TCGAATAGCGGGACCAAAAC	ACTTGAATAAAGGGCAGAGTGA	50.0	40.9	58.3	57.4	1499-1588 bp	1.0	62.4
trnQ-UUG-psbK_p3	CTTCGAATAGCGGGACCAAA	ACTTGAATAAAGGGCAGAGTGAA	50.0	39.1	58.0	58.0	1501-1590 bp	1.0	61.4
trnQ-UUG-psbK_p4	GAACCTTCGAATAGCGGGAC	ACTTGAATAAAGGGCAGAGTGA	55.0	40.9	58.4	57.4	1505-1594 bp	1.0	61.1
trnQ-UUG-psbK_p5	TTCGAATAGCGGGACCAAAA	ACTTGAATAAAGGGCAGAGTGA	45.0	40.9	57.5	57.4	1500-1589 bp	1.0	60.7