Hotspot

trnK-UUU-rps16

LSC · 4473-6210 · 1738 bp

Back to Myrocarpus

Pi 0.0324

MarkerSeek score 66.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1738
variable_sites: 55
pi: 0.0324
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGCCGAGTACTCTACCATTGA	AAGGCGGGTGTTTTTATGGA	47.6	45.0	58.0	57.7	1824-2189 bp	1.0	73.3
trnK-UUU-rps16_p2	AGCCGAGTACTCTACCATTGA	AGGCGGGTGTTTTTATGGAA	47.6	45.0	58.0	57.7	1823-2188 bp	1.0	73.3
trnK-UUU-rps16_p3	AGCCGAGTACTCTACCATTGA	GGCGGGTGTTTTTATGGAAC	47.6	50.0	58.0	57.6	1822-2187 bp	1.0	72.8
trnK-UUU-rps16_p4	GCCGAGTACTCTACCATTGAG	AAGGCGGGTGTTTTTATGGA	52.4	45.0	57.6	57.7	1823-2188 bp	1.0	70.6
trnK-UUU-rps16_p5	GCCGAGTACTCTACCATTGAG	AGGCGGGTGTTTTTATGGAA	52.4	45.0	57.6	57.7	1822-2187 bp	1.0	70.6