Hotspot

trnH-GUG-psbA

LSC · 120-520 · 401 bp

Back to Myrocarpus

Pi 0.0157
MarkerSeek score 67.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
401
variable_sites
6
pi
0.0157
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 470-500 bp 1.0 82.3
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TAACCGGGCTAACCTTGGTA 55.0 50.0 58.1 58.1 522-552 bp 1.0 82.1
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA TAACCGGGCTAACCTTGGTA 50.0 50.0 57.9 58.1 536-566 bp 1.0 82.0
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC TAACCGGGCTAACCTTGGTA 50.0 50.0 57.9 58.1 535-565 bp 1.0 82.0
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC TAACCGGGCTAACCTTGGTA 50.0 50.0 57.9 58.1 537-567 bp 1.0 82.0