Hotspot

psbE-petL

LSC · 67701-68896 · 1196 bp

Back to Myristica

Pi 0.00201

MarkerSeek score 52.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1196
variable_sites: 5
pi: 0.00201
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCA	50.0	45.0	57.9	57.3	1342-1343 bp	1.0	74.7
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCAGCCAGTAGAAAACCAA	50.0	45.0	57.9	57.3	1341-1342 bp	1.0	74.7
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCA	45.0	45.0	58.2	57.3	1351-1352 bp	1.0	73.7
psbE-petL_p4	ACGAATAACCAACCCGCAAT	AGCAGCCAGTAGAAAACCAA	45.0	45.0	58.2	57.3	1350-1351 bp	1.0	73.7
psbE-petL_p5	CGAATAACCAACCCGCAATG	AGCAGCCAGTAGAAAACCAA	50.0	45.0	57.8	57.3	1349-1350 bp	1.0	73.6