Hotspot

accD-psaI

LSC · 61563-62195 · 633 bp

Back to Myristica

Pi 0.00506

MarkerSeek score 48.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 633
variable_sites: 7
pi: 0.00506
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCACAAGCGGCTGAGTATTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	847-852 bp	1.0	80.5
accD-psaI_p2	TCACTCGGAAGAAGAACCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1331-1336 bp	1.0	80.3
accD-psaI_p3	TGGGATCTGTAGTAGGCGAG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1182-1187 bp	1.0	80.3
accD-psaI_p4	TCACAAGCGGCTGAGTATTC	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.0	58.0	838-843 bp	1.0	80.3
accD-psaI_p5	TCACTCGGAAGAAGAACCCT	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.0	58.0	1322-1327 bp	1.0	80.1