Hotspot

psaA-ycf3

LSC · 43665-44323 · 659 bp

Back to Myristica

Pi 0.00244

MarkerSeek score 54.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 659
variable_sites: 4
pi: 0.00244
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TGAAGATCACAAGGCGGTTC	50.0	50.0	57.9	58.2	760-765 bp	1.0	78.5
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACAAGGCGGTT	50.0	45.0	57.9	57.7	761-766 bp	1.0	77.9
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	GTCCGATCAAGCTGCTGAAT	50.0	50.0	57.9	58.3	837-842 bp	1.0	77.2
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	806-811 bp	1.0	77.1
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	GTTGAAGATCACAAGGCGGT	50.0	50.0	57.9	58.5	762-767 bp	1.0	76.2