Hotspot

trnE-UUC-trnT-GGU

LSC · 32561-33153 · 593 bp

Back to Myristica

Pi 0.00202

MarkerSeek score 55.60

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 593
variable_sites: 3
pi: 0.00202
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	694-696 bp	1.0	78.4
trnE-UUC-trnT-GGU_p2	GCTGCCTCCTTGAAAGAGAG	GACTGAAGACTGGAGCTTCG	55.0	55.0	58.3	58.0	738-740 bp	1.0	78.3
trnE-UUC-trnT-GGU_p3	CGTAGTACCCTACCTACCCC	GAACCGATGACTTACGCCTT	60.0	50.0	57.7	58.0	733-735 bp	1.0	78.3
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	673-675 bp	1.0	78.3
trnE-UUC-trnT-GGU_p5	CGTAGTACCCTACCTACCCC	GACTGAAGACTGGAGCTTCG	60.0	55.0	57.7	58.0	777-779 bp	1.0	78.2