Hotspot

psbM-trnD-GUC

LSC · 30886-31877 · 992 bp

Back to Myristica

Pi 0.00262

MarkerSeek score 60.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 992
variable_sites: 6
pi: 0.00262
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1102-1107 bp	1.0	77.7
psbM-trnD-GUC_p2	TGAACAGCGCAGTAGCAATA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	57.6	57.9	1101-1106 bp	1.0	76.7
psbM-trnD-GUC_p3	AATGAACAGCGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.5	57.9	1103-1108 bp	1.0	75.9
psbM-trnD-GUC_p4	AGAATGAACAGCGCAGTAGC	AGTTCAATCGGTCAGAGCAC	50.0	50.0	58.6	57.9	1105-1110 bp	1.0	74.7
psbM-trnD-GUC_p5	ACTAGAATGAACAGCGCAGT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	57.2	57.9	1108-1113 bp	1.0	73.7