Hotspot

trnS-GCU-trnG-UCC

LSC · 8455-9348 · 894 bp

Back to Myristica

Pi 0.00336

MarkerSeek score 60.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 894
variable_sites: 7
pi: 0.00336
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGTCGCTTTAGTCCA	AATCAAACCGAAAGACCCCC	50.0	50.0	58.0	58.1	1005-1006 bp	1.0	80.0
trnS-GCU-trnG-UCC_p2	AATCCGTCGCTTTAGTCCAC	AATCAAACCGAAAGACCCCC	50.0	50.0	58.0	58.1	1004-1005 bp	1.0	79.9
trnS-GCU-trnG-UCC_p3	CAAGTGATCAGAGGGAACGG	AATCAAACCGAAAGACCCCC	55.0	50.0	58.0	58.1	1075-1076 bp	1.0	79.8
trnS-GCU-trnG-UCC_p4	CAATCCGTCGCTTTAGTCCA	AACCGAAAGACCCCCTAACT	50.0	50.0	58.0	58.3	1000-1001 bp	1.0	78.5
trnS-GCU-trnG-UCC_p5	AATCCGTCGCTTTAGTCCAC	AACCGAAAGACCCCCTAACT	50.0	50.0	58.0	58.3	999-1000 bp	1.0	78.5