Hotspot

trnH-GUG-psbA

LSC · 79-379 · 301 bp

Back to Myristica

Pi 0.0153
MarkerSeek score 63.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
301
variable_sites
9
pi
0.0153
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TGCTCACAATTTCCCTCTGG 55.0 50.0 58.1 57.8 381-386 bp 1.0 79.6
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TGCTCACAATTTCCCTCTGG 50.0 50.0 57.9 57.8 395-400 bp 1.0 79.5
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TGCTCACAATTTCCCTCTGG 50.0 50.0 57.9 57.8 394-399 bp 1.0 79.5
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TGCTCACAATTTCCCTCTGG 50.0 50.0 57.9 57.8 396-401 bp 1.0 79.5
trnH-GUG-psbA_p5 GCGAATGACGGGAATTGAAC TGCTCACAATTTCCCTCTGG 50.0 50.0 57.8 57.8 433-438 bp 1.0 79.0