Hotspot

chlL-trnN-GUU

IRa · 129964-130708 · 745 bp

Back to Myriopteris

Pi 0.169

MarkerSeek score 75.80

Primer available yes

Label chlL-trnN-GUU

Feature Metadata

label_name: chlL-trnN-GUU
feature_type: igs
region: IRa
length_bp: 745
variable_sites: 77
pi: 0.169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.61
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL-trnN-GUU_p1	TCCATAAACTGCTATTTTCGT	AGGATTCGAACCTACGACCA	33.3	50.0	53.2	58.2	539-828 bp	1.0	52.0
chlL-trnN-GUU_p2	TTCCATAAACTGCTATTTTCGT	AGGATTCGAACCTACGACCA	31.8	50.0	54.1	58.2	540-829 bp	1.0	52.0
chlL-trnN-GUU_p3	TCCATAAACTGCTATTTTCGT	CCGGGTAGGATTCGAACCTA	33.3	55.0	53.2	58.3	545-834 bp	1.0	52.0
chlL-trnN-GUU_p4	TTCCATAAACTGCTATTTTCGT	CCGGGTAGGATTCGAACCTA	31.8	55.0	54.1	58.3	546-835 bp	1.0	52.0
chlL-trnN-GUU_p5	TTTCCATAAACTGCTATTTTCGT	AGGATTCGAACCTACGACCA	30.4	50.0	54.9	58.2	541-830 bp	1.0	52.0