Hotspot

trnP-GGG-trnL-UAG

SSC · 112492-112679 · 188 bp

Back to Myriopteris

Pi 0.128

MarkerSeek score 83.10

Primer available yes

Label trnP-GGG-trnL-UAG

Feature Metadata

label_name: trnP-GGG-trnL-UAG
feature_type: igs
region: SSC
length_bp: 188
variable_sites: 23
pi: 0.128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-trnL-UAG_p1	TGAACCTGTGACCTCCATCA	AGAGCAGCGTGTCTACCTAT	50.0	50.0	58.3	57.9	272-280 bp	1.0	86.3
trnP-GGG-trnL-UAG_p2	TGAACCTGTGACCTCCATCA	CTCTGGCACTGCTTCCTAAG	50.0	55.0	58.3	58.0	290-298 bp	1.0	86.1
trnP-GGG-trnL-UAG_p3	TGAACCTGTGACCTCCATCA	CTGGCACTGCTTCCTAAGAG	50.0	55.0	58.3	58.0	288-296 bp	1.0	86.1
trnP-GGG-trnL-UAG_p4	TGAACCTGTGACCTCCATCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.3	57.9	277-285 bp	1.0	85.7
trnP-GGG-trnL-UAG_p5	TGAACCTGTGACCTCCATCA	AAGAGCAGCGTGTCTACCTA	50.0	50.0	58.3	58.2	273-281 bp	1.0	85.5