Hotspot

clpP1-psbB

LSC · 67908-68415 · 508 bp

Back to Myriopteris

Pi 0.0701

MarkerSeek score 78.60

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: LSC
length_bp: 508
variable_sites: 35
pi: 0.0701
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	CGAAAGGGCACTTTTGGAAC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.9	58.2	558-560 bp	1.0	83.3
clpP1-psbB_p2	CTATACGTCAACCCGAACCG	CGATACCAAGGCAAACCCAT	55.0	50.0	58.2	58.2	598-600 bp	1.0	82.6
clpP1-psbB_p3	CGAAAGGGCACTTTTGGAAC	TAATACGACGGTGTGAACGC	50.0	50.0	57.9	58.4	577-579 bp	1.0	81.9
clpP1-psbB_p4	CGACATACCTAACGAGTCGC	CGATACCAAGGCAAACCCAT	55.0	50.0	58.3	58.2	619-621 bp	1.0	81.7
clpP1-psbB_p5	GCACTATACGTCAACCCGAA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.7	58.2	601-603 bp	1.0	81.7