Hotspot

petA-psbJ

LSC · 59649-60660 · 1012 bp

Back to Myriopteris

Pi 0.112

MarkerSeek score 81.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1012
variable_sites: 111
pi: 0.112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGTTGGGGGATTTGGTCAAG	TTAGCTTACGACGTTTCCGG	50.0	50.0	58.0	58.0	1825-1834 bp	1.0	86.5
petA-psbJ_p2	TGTTGGGGGATTTGGTCAAG	GGCTGGCTGTCTGTAAGTAC	50.0	55.0	58.0	58.0	1849-1858 bp	1.0	86.4
petA-psbJ_p3	GCAGATCAGCCGTTGACTAA	GGCTGGCTGTCTGTAAGTAC	50.0	55.0	58.0	58.0	1878-1887 bp	1.0	86.4
petA-psbJ_p4	GCAGATCAGCCGTTGACTAA	TTAGCTTACGACGTTTCCGG	50.0	50.0	58.0	58.0	1854-1863 bp	1.0	86.4
petA-psbJ_p5	CAGCGACGGGAAGGATAAAA	GGCTGGCTGTCTGTAAGTAC	50.0	55.0	58.0	58.0	2027-2036 bp	1.0	86.1