Hotspot

trnS-GGA-rps4

LSC · 43605-44054 · 450 bp

Back to Myriopteris

Pi 0.122
MarkerSeek score 81.40
Primer available yes
Label trnS-GGA-rps4

Feature Metadata

label_name
trnS-GGA-rps4
feature_type
igs
region
LSC
length_bp
450
variable_sites
54
pi
0.122
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-rps4_p1 GTTCGAATCCCTCCCCCT TGAGTATCATTCCCGAAAAGCT 61.1 40.9 57.9 57.5 488-496 bp 1.0 52.9
trnS-GGA-rps4_p2 GTTCGAATCCCTCCCCCTC TGAGTATCATTCCCGAAAAGCT 63.2 40.9 59.2 57.5 488-496 bp 1.0 52.0
trnS-GGA-rps4_p3 TTCGAATCCCTCCCCCTC TGAGTATCATTCCCGAAAAGCT 61.1 40.9 57.6 57.5 487-495 bp 1.0 50.2
trnS-GGA-rps4_p4 GTTCGAATCCCTCCCCCT TGAGTATCATTCCCGAAAAGC 61.1 42.9 57.9 56.0 488-496 bp 1.0 49.3
trnS-GGA-rps4_p5 GTTCGAATCCCTCCCCCTC TGAGTATCATTCCCGAAAAGC 63.2 42.9 59.2 56.0 488-496 bp 1.0 49.3