Hotspot

psbM-petN

LSC · 28797-29723 · 927 bp

Back to Myriopteris

Pi 0.0929
MarkerSeek score 79.00
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
LSC
length_bp
927
variable_sites
85
pi
0.0929
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 AAACAGCCGCTCGGAATAAC CGTTTTCCCTCTCGCTTGTA 50.0 50.0 58.9 57.9 997-999 bp 1.0 79.9
psbM-petN_p2 AAACAGCCGCTCGGAATAAC TACGTTTTCCCTCTCGCTTG 50.0 50.0 58.9 57.9 999-1001 bp 1.0 79.9
psbM-petN_p3 CAAACAGCCGCTCGGAATAA CGTTTTCCCTCTCGCTTGTA 50.0 50.0 58.9 57.9 998-1000 bp 1.0 79.9
psbM-petN_p4 CAAACAGCCGCTCGGAATAA TACGTTTTCCCTCTCGCTTG 50.0 50.0 58.9 57.9 1000-1002 bp 1.0 79.9
psbM-petN_p5 TACATTCAAACAGCCGCTCG CGTTTTCCCTCTCGCTTGTA 50.0 50.0 58.9 57.9 1004-1006 bp 1.0 79.8