Hotspot

rpoB-trnD-GUC

LSC · 26880-27741 · 862 bp

Back to Myriopteris

Pi 0.0813

MarkerSeek score 81.40

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: LSC
length_bp: 862
variable_sites: 69
pi: 0.0813
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	TGCAAGTTCTGGTAGTACGG	GCGGTGCTCTAACCAATTGA	50.0	50.0	57.3	58.6	917-920 bp	1.0	76.8
rpoB-trnD-GUC_p2	GCAAGTTCTGGTAGTACGGT	GCGGTGCTCTAACCAATTGA	50.0	50.0	57.3	58.6	916-919 bp	1.0	76.8
rpoB-trnD-GUC_p3	TTCGTGCAAGTTCTGGTAGT	GCGGTGCTCTAACCAATTGA	45.0	50.0	57.1	58.6	921-924 bp	1.0	75.5
rpoB-trnD-GUC_p4	TCGTGCAAGTTCTGGTAGTAC	GCGGTGCTCTAACCAATTGA	47.6	50.0	57.7	58.6	920-923 bp	1.0	72.4
rpoB-trnD-GUC_p5	TGCAAGTTCTGGTAGTACGG	GGCGGTGCTCTAACCAATT	50.0	52.6	57.3	58.1	918-921 bp	1.0	72.2