Hotspot

trnS-GCU-psb30

LSC · 8048-8753 · 706 bp

Back to Myriopteris

Pi 0.125

MarkerSeek score 82.60

Primer available yes

Label trnS-GCU-psb30

Feature Metadata

label_name: trnS-GCU-psb30
feature_type: igs
region: LSC
length_bp: 706
variable_sites: 87
pi: 0.125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psb30_p1	GCAATCCGACGCTTTAAACC	GCGAGTTGCGTAATTATTTCCA	50.0	40.9	58.1	57.7	771-806 bp	1.0	70.0
trnS-GCU-psb30_p2	TAAACCTCTCGGCCATCTCT	GCGAGTTGCGTAATTATTTCCA	50.0	40.9	57.8	57.7	757-792 bp	1.0	69.6
trnS-GCU-psb30_p3	GCTTTAAACCTCTCGGCCAT	GCGAGTTGCGTAATTATTTCCA	50.0	40.9	58.2	57.7	761-796 bp	1.0	68.9
trnS-GCU-psb30_p4	TCCGACGCTTTAAACCTCTC	GCGAGTTGCGTAATTATTTCCA	50.0	40.9	57.6	57.7	767-802 bp	1.0	68.0
trnS-GCU-psb30_p5	GCAATCCGACGCTTTAAACC	GCGAGTTGCGTAATTATTTCC	50.0	42.9	58.1	56.0	771-806 bp	1.0	64.5