Hotspot

psbK-psbI

LSC · 7224-7770 · 547 bp

Back to Myriopteris

Pi 0.0996

MarkerSeek score 85.70

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 53
pi: 0.0996
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCCGCAGTTAGTTTTCG	TCCAAAAACGAAAAGCGAAACA	50.0	36.4	58.0	58.2	627-637 bp	1.0	70.9
psbK-psbI_p2	AGCCGCAGTTAGTTTTCGAT	TCCAAAAACGAAAAGCGAAACA	45.0	36.4	57.9	58.2	625-635 bp	1.0	70.6
psbK-psbI_p3	AAGCCGCAGTTAGTTTTCGA	TCCAAAAACGAAAAGCGAAACA	45.0	36.4	58.1	58.2	626-636 bp	1.0	70.3
psbK-psbI_p4	CAAGCCGCAGTTAGTTTTCG	CCAAAAACGAAAAGCGAAACA	50.0	38.1	58.0	56.8	626-636 bp	1.0	70.2
psbK-psbI_p5	GCCAAGTTACCCGAAGCTTA	TCCAAAAACGAAAAGCGAAACA	50.0	36.4	57.9	58.2	720-730 bp	1.0	69.4