Hotspot

matK-rps16

LSC · 2994-3700 · 707 bp

Back to Myriopteris

Pi 0.118

MarkerSeek score 81.70

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 81
pi: 0.118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	CCTCAGAACCCAACTCGATG	GGGTTCATCTTTGCATGCAC	55.0	50.0	58.0	58.0	1118 bp	0.5	59.9
matK-rps16_p2	CCTCAGAACCCAACTCGATG	GGTTCATCTTTGCATGCACC	55.0	50.0	58.0	58.0	1117 bp	0.5	59.9
matK-rps16_p3	GTTTGGCCCATGTAATTGCC	GGGTTCATCTTTGCATGCAC	50.0	50.0	58.0	58.0	1099 bp	0.5	59.8
matK-rps16_p4	GTTTGGCCCATGTAATTGCC	GGTTCATCTTTGCATGCACC	50.0	50.0	58.0	58.0	1098 bp	0.5	59.8
matK-rps16_p5	CCTCAGAACCCAACTCGATG	CGCACGAACTGAAACTTTCC	55.0	50.0	58.0	58.0	1155 bp	0.5	59.7