Hotspot

rpl32-trnL-UAG

SSC · 119311-120540 · 1230 bp

Back to Myriophyllum

Pi 0.101

MarkerSeek score 84.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1230
variable_sites: 211
pi: 0.101
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACCGGCAATTCAAAAAGTTT	AGCGTGTCTACCGATTTCAC	35.0	50.0	54.9	58.0	1073-1328 bp	1.0	66.8
rpl32-trnL-UAG_p2	ACCGGCAATTCAAAAAGTTT	CAGCGTGTCTACCGATTTCA	35.0	50.0	54.9	58.0	1074-1329 bp	1.0	66.8
rpl32-trnL-UAG_p3	ACCGGCAATTCAAAAAGTTT	TCCTAAGAGCAGCGTGTCTA	35.0	50.0	54.9	57.9	1083-1338 bp	1.0	65.9
rpl32-trnL-UAG_p4	ACCGGCAATTCAAAAAGTTTT	AGCGTGTCTACCGATTTCAC	33.3	50.0	55.7	58.0	1073-1328 bp	1.0	65.4
rpl32-trnL-UAG_p5	ACCGGCAATTCAAAAAGTTTT	CAGCGTGTCTACCGATTTCA	33.3	50.0	55.7	58.0	1074-1329 bp	1.0	65.4