Hotspot

rbcL-accD

LSC · 61303-61999 · 697 bp

Back to Myriophyllum

Pi 0.0572

MarkerSeek score 83.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 697
variable_sites: 82
pi: 0.0572
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTGCAAGTGGAGTCCTGAAC	CTAACCGGATCGTCACTTCC	50.0	55.0	58.0	57.8	907-934 bp	0.6	70.2
rbcL-accD_p2	GAGGAACCTTAGGACACCCT	CTAACCGGATCGTCACTTCC	55.0	55.0	58.0	57.8	1040-1067 bp	0.6	69.3
rbcL-accD_p3	AAAGAGCCCAATTTGCCAGA	CTAACCGGATCGTCACTTCC	45.0	55.0	58.0	57.8	1496-1523 bp	0.6	67.9
rbcL-accD_p4	CTTAACCGGGGGATTCACTG	CTAACCGGATCGTCACTTCC	55.0	55.0	58.0	57.8	1444-1471 bp	0.6	67.8
rbcL-accD_p5	GCATGACTACTTAACCGGGG	CTAACCGGATCGTCACTTCC	55.0	55.0	58.1	57.8	1453-1480 bp	0.6	67.7