Hotspot

trnF-GAA-ndhJ

LSC · 52378-53090 · 713 bp

Back to Myriophyllum

Pi 0.0504

MarkerSeek score 90.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 713
variable_sites: 69
pi: 0.0504
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 90.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	805-847 bp	1.0	89.6
trnF-GAA-ndhJ_p2	ATAGCCGGGATAGCTCAGTT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.0	58.1	847-889 bp	1.0	89.5
trnF-GAA-ndhJ_p3	GGTATAGCCGGGATAGCTCA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.2	58.1	850-892 bp	1.0	88.4
trnF-GAA-ndhJ_p4	ATAGCCGGGATAGCTCAGTT	TCATAATCATCCCCGCCTGA	50.0	50.0	58.0	58.3	887-929 bp	1.0	87.8
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TCATAATCATCCCCGCCTGA	50.0	50.0	58.1	58.3	845-887 bp	1.0	87.8