Hotspot

trnT-GGU-psbD

LSC · 34279-35816 · 1538 bp

Back to Myriophyllum

Pi 0.049

MarkerSeek score 85.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1538
variable_sites: 137
pi: 0.049
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 85.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1592-1657 bp	1.0	88.4
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1600-1665 bp	1.0	86.5
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	CGAAACGGTCCCTACGTAAC	50.0	55.0	58.3	58.4	1593-1658 bp	1.0	85.4
trnT-GGU-psbD_p4	GCCATGGTAAGGCGTAAGTC	GAAACGGTCCCTACGTAACC	55.0	55.0	58.7	58.0	1602-1667 bp	1.0	85.0
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GGTCCCTACGTAACCAGTCA	50.0	55.0	58.3	58.5	1587-1652 bp	1.0	84.9