Hotspot

trnD-GUC-psbM

LSC · 30274-31244 · 971 bp

Back to Myriophyllum

Pi 0.0622

MarkerSeek score 86.30

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 971
variable_sites: 105
pi: 0.0622
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	CCTGTCAAGGCGGAAGTTG	AGAATAAACAGTGCAGTAGC	57.9	40.0	58.8	53.3	864-1119 bp	1.0	52.0
trnD-GUC-psbM_p2	AGTTCAATTGGTGAGAGCAC	AGAATAAACAGTGCAGTAGC	45.0	40.0	56.0	53.3	887-1142 bp	1.0	52.0
trnD-GUC-psbM_p3	TGTCAAGGCGGAAGTTGC	AGAATAAACAGTGCAGTAGC	55.6	40.0	58.3	53.3	862-1117 bp	1.0	52.0
trnD-GUC-psbM_p4	TGTAGTTCAATTGGTGAGAGCA	AGAATAAACAGTGCAGTAGC	40.9	40.0	57.6	53.3	890-1145 bp	1.0	52.0
trnD-GUC-psbM_p5	CTGTCAAGGCGGAAGTTGC	AGAATAAACAGTGCAGTAGC	57.9	40.0	59.4	53.3	863-1118 bp	1.0	52.0