Hotspot

trnY-GUA-trnD-GUC

LSC · 29957-30199 · 243 bp

Back to Myriophyllum

Pi 0.0604
MarkerSeek score 83.40
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
243
variable_sites
27
pi
0.0604
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 GGCAATATGTCTACGCTGGT CAACTTCCGCCTTGACAGG 50.0 57.9 57.8 58.8 331-342 bp 1.0 74.3
trnY-GUA-trnD-GUC_p2 TGGCAATATGTCTACGCTGG CAACTTCCGCCTTGACAGG 50.0 57.9 57.8 58.8 332-343 bp 1.0 74.2
trnY-GUA-trnD-GUC_p3 GGGGACGGACTGTAAATTCG CAACTTCCGCCTTGACAGG 55.0 57.9 58.4 58.8 353-364 bp 1.0 72.7
trnY-GUA-trnD-GUC_p4 GGGACGGACTGTAAATTCGT CAACTTCCGCCTTGACAGG 50.0 57.9 57.6 58.8 352-363 bp 1.0 72.6
trnY-GUA-trnD-GUC_p5 CGGACTGTAAATTCGTTGGC CAACTTCCGCCTTGACAGG 50.0 57.9 57.5 58.8 348-359 bp 1.0 71.5