Hotspot

rps16-trnQ-UUG

LSC · 6493-8508 · 2016 bp

Back to Myriophyllum

Pi 0.124
MarkerSeek score 82.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
2016
variable_sites
411
pi
0.124
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.94
markerseek_score
82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CTCCACAGCTGATCATGTCC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.0 58.1 1630-2112 bp 1.0 91.0
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1607-2089 bp 1.0 90.3
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1618-2100 bp 1.0 89.8
rps16-trnQ-UUG_p4 CCACAGCTGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 1628-2110 bp 1.0 88.1
rps16-trnQ-UUG_p5 TCCACAGCTGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 1629-2111 bp 1.0 88.0