Hotspot

trnK-UUU-rps16

LSC · 4484-5379 · 896 bp

Back to Myriophyllum

Pi 0.0696
MarkerSeek score 84.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
896
variable_sites
116
pi
0.0696
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AACCCAACCGCTCTTTTGAT TTTTCTTGAGCCGTACGAGG 45.0 50.0 58.0 57.9 1752-1783 bp 1.0 90.0
trnK-UUU-rps16_p2 ACCCAACCGCTCTTTTGATT TTTTCTTGAGCCGTACGAGG 45.0 50.0 58.0 57.9 1751-1782 bp 1.0 90.0
trnK-UUU-rps16_p3 AACCCAACCGCTCTTTTGAT GCCGTACGAGGAGAAAACTT 45.0 50.0 58.0 57.9 1743-1774 bp 1.0 90.0
trnK-UUU-rps16_p4 ACCCAACCGCTCTTTTGATT GCCGTACGAGGAGAAAACTT 45.0 50.0 58.0 57.9 1742-1773 bp 1.0 90.0
trnK-UUU-rps16_p5 ACACCTCGGAGACAGGTAAA TTTTCTTGAGCCGTACGAGG 50.0 50.0 58.0 57.9 1908-1939 bp 0.6 68.1