Hotspot

clpP

LSC · 70389-72429 · 2041 bp

Back to Myricaria

Pi 0.00802

MarkerSeek score 49.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2041
variable_sites: 38
pi: 0.00802
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GATTGGCTGTCTTGCGTTTC	CTGGCTATGGAATGAACCCC	50.0	55.0	58.0	58.0	2621-2634 bp	1.0	81.4
clpP_p2	GATTGGCTGTCTTGCGTTTC	CGGCTACACCTTCGTAACTC	50.0	55.0	58.0	58.1	2975-2988 bp	1.0	80.6
clpP_p3	GATTGGCTGTCTTGCGTTTC	CCTGTAATACTCCAACCGCC	50.0	55.0	58.0	58.1	2928-2941 bp	0.857143	75.1
clpP_p4	GATTGGCTGTCTTGCGTTTC	CTGTAATACTCCAACCGCCC	50.0	55.0	58.0	58.1	2927-2940 bp	0.857143	75.1
clpP_p5	GATTGGCTGTCTTGCGTTTC	GAACCCCCTTGTTCCGAAAT	50.0	50.0	58.0	58.1	2608-2621 bp	0.857143	75.0