Hotspot

trnM-CAU-atpE

LSC · 53562-53848 · 287 bp

Back to Myricaria

Pi 0.0156

MarkerSeek score 60.20

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 287
variable_sites: 11
pi: 0.0156
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	AATTCAAGCGATGGGTTCCA	ACTTGAGGAAAGCAGATGGC	45.0	50.0	57.8	58.2	493-499 bp	1.0	79.7
trnM-CAU-atpE_p2	TCAAGCGATGGGTTCCATTT	ACTTGAGGAAAGCAGATGGC	45.0	50.0	57.8	58.2	490-496 bp	1.0	79.7
trnM-CAU-atpE_p3	TTCAAGCGATGGGTTCCATT	ACTTGAGGAAAGCAGATGGC	45.0	50.0	57.8	58.2	491-497 bp	1.0	79.7
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	ACTTGAGGAAAGCAGATGGC	50.0	50.0	58.4	58.2	424-430 bp	1.0	78.6
trnM-CAU-atpE_p5	GTATTGCTTTCATACGGCGG	ACTTGAGGAAAGCAGATGGC	50.0	50.0	57.3	58.2	425-431 bp	1.0	76.5