Hotspot

psaA-ycf3

LSC · 42394-43243 · 850 bp

Back to Myricaria

Pi 0.0102

MarkerSeek score 67.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 850
variable_sites: 24
pi: 0.0102
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCACTCCTCAAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.7	58.0	1022-1028 bp	1.0	79.8
psaA-ycf3_p2	GCCCACTCCTCAAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.7	58.0	1004-1010 bp	1.0	79.8
psaA-ycf3_p3	GCCCACTCCTCAAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.7	58.1	1024-1030 bp	1.0	79.0
psaA-ycf3_p4	GCCCACTCCTCAAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.7	57.8	951-957 bp	1.0	79.0
psaA-ycf3_p5	GCCCACTCCTCAAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.7	58.2	955-961 bp	1.0	78.7