Hotspot

petN-psbM

LSC · 28817-29691 · 875 bp

Back to Myricaria

Pi 0.0115

MarkerSeek score 69.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 28
pi: 0.0115
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	ACGTAAAAACGGTCAGCCAA	50.0	45.0	58.2	58.3	986-997 bp	1.0	79.1
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	ACGTAAAAACGGTCAGCCAA	50.0	45.0	57.6	58.3	925-936 bp	1.0	77.7
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TACGTAAAAACGGTCAGCCA	50.0	45.0	58.2	57.5	987-998 bp	1.0	77.6
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TACGTAAAAACGGTCAGCCA	50.0	45.0	57.6	57.5	926-937 bp	1.0	76.2
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	CGTAAAAACGGTCAGCCAAA	50.0	45.0	58.2	57.2	985-996 bp	1.0	75.7