Hotspot

rpoB-trnC-GCA

LSC · 26650-27906 · 1257 bp

Back to Myricaria

Pi 0.0104

MarkerSeek score 61.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1257
variable_sites: 33
pi: 0.0104
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCCTCATTTTCATCCGGGAG	CCGGATTTGAACTGGGGAAT	55.0	50.0	58.0	57.9	1321-1351 bp	1.0	82.6
rpoB-trnC-GCA_p2	CTCATTTTCATCCGGGAGCA	CCGGATTTGAACTGGGGAAT	50.0	50.0	58.0	57.9	1319-1349 bp	1.0	82.5
rpoB-trnC-GCA_p3	CCCTCATTTTCATCCGGGAG	ATTTGCAATCCTCCGCCTTA	55.0	45.0	58.0	57.6	1296-1326 bp	1.0	80.4
rpoB-trnC-GCA_p4	CTCATTTTCATCCGGGAGCA	ATTTGCAATCCTCCGCCTTA	50.0	45.0	58.0	57.6	1294-1324 bp	1.0	80.3
rpoB-trnC-GCA_p5	TCCCTCATTTTCATCCGGGA	CCGGATTTGAACTGGGGAAT	50.0	50.0	58.4	57.9	1322-1352 bp	1.0	79.6