Hotspot

trnS-GCU-trnG-UCC

LSC · 8172-9070 · 899 bp

Back to Myricaria

Pi 0.0178

MarkerSeek score 70.40

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 899
variable_sites: 41
pi: 0.0178
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCCTCGGTACAAATGACTCG	AATCAAACCGAAAGACCCCT	55.0	45.0	58.1	57.0	1016-1056 bp	1.0	78.8
trnS-GCU-trnG-UCC_p2	CCCTCGGTACAAATGACTCG	ATCAAACCGAAAGACCCCTT	55.0	45.0	58.1	57.0	1015-1055 bp	1.0	78.8
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	AATCAAACCGAAAGACCCCT	50.0	45.0	58.2	57.0	1037-1077 bp	1.0	78.1
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	ATCAAACCGAAAGACCCCTT	50.0	45.0	58.2	57.0	1036-1076 bp	1.0	78.1
trnS-GCU-trnG-UCC_p5	CCCTCGGTACAAATGACTCG	ACCGAAAGACCCCTTAACTC	55.0	50.0	58.1	56.9	1010-1050 bp	1.0	77.4