Hotspot

rps16-trnQ-UUG

LSC · 5949-6868 · 920 bp

Back to Myricaria

Pi 0.0137

MarkerSeek score 64.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 920
variable_sites: 30
pi: 0.0137
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	981-1003 bp	1.0	82.1
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	992-1014 bp	1.0	81.6
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GTGTCGAATAGAAGTGGGGC	45.0	55.0	58.1	58.3	1043-1065 bp	1.0	79.7
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	GTGTCGAATAGAAGTGGGGC	50.0	55.0	58.2	58.3	1054-1076 bp	1.0	79.2
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	985-1007 bp	1.0	77.4