Hotspot

ndhG

SSC · 117238-117771 · 534 bp

Back to Myosurus

Pi 0.00562

MarkerSeek score 47.60

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 534
variable_sites: 3
pi: 0.00562
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ATTTTTATTTCGAAGTAGTCAGT	CCGTATCAACACCCTAGCCT	26.1	55.0	52.2	58.9	679 bp	1.0	40.4
ndhG_p2	ATTTTTATTTCGAAGTAGTCAGT	TCAACACCCTAGCCTATTCGA	26.1	47.6	52.2	58.3	674 bp	1.0	40.4
ndhG_p3	ATTTTTATTTCGAAGTAGTCAGT	CAACACCCTAGCCTATTCGA	26.1	50.0	52.2	56.7	673 bp	1.0	40.4
ndhG_p4	ATTTTTATTTCGAAGTAGTCAGT	TCAACACCCTAGCCTATTCG	26.1	50.0	52.2	56.7	674 bp	1.0	40.4
ndhG_p5	ATTTTTATTTCGAAGTAGTCAGT	CCGTATCAACACCCTAGCC	26.1	57.9	52.2	57.3	679 bp	1.0	40.4