Hotspot

ccsA

SSC · 113369-114340 · 972 bp

Back to Myosurus

Pi 0.00617

MarkerSeek score 52.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 6
pi: 0.00617
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TCTGGATTTCTATTCCATTGT	TCGTTCTATAGGCTATTATTTCGA	33.3	33.3	52.2	55.1	1078 bp	1.0	40.4
ccsA_p2	TCTGGATTTCTATTCCATTGTT	TCGTTCTATAGGCTATTATTTCGA	31.8	33.3	53.1	55.1	1078 bp	1.0	40.4
ccsA_p3	TTCTGGATTTCTATTCCATTGT	TCGTTCTATAGGCTATTATTTCGA	31.8	33.3	53.1	55.1	1079 bp	1.0	40.4
ccsA_p4	TTCTGGATTTCTATTCCATTGTT	TCGTTCTATAGGCTATTATTTCGA	30.4	33.3	54.0	55.1	1079 bp	1.0	40.4
ccsA_p5	TCTGGATTTCTATTCCATTGT	TCGTTCTATAGGCTATTATTTCGATCT	33.3	33.3	52.2	57.8	1078 bp	1.0	40.4