Hotspot

rps15-ycf1

SSC · 122408-122783 · 376 bp

Back to Myosurus

Pi 0.00802
MarkerSeek score 44.30
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
376
variable_sites
3
pi
0.00802
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 CCCAGAATCTTGCGTAGACC CCTTATTGAGCCACTCCGAC 55.0 55.0 58.1 58.1 960-961 bp 1.0 80.0
rps15-ycf1_p2 CCAATATGGGTTCGCAGTCA CCTTATTGAGCCACTCCGAC 50.0 55.0 58.0 58.1 1339-1340 bp 1.0 79.9
rps15-ycf1_p3 CCAATATGGGTTCGCAGTCA GGTTGGCGATCAAAACCAAG 50.0 50.0 58.0 57.9 1117-1118 bp 1.0 79.9
rps15-ycf1_p4 AACACCATGCATTGATGGGT CCTTATTGAGCCACTCCGAC 45.0 55.0 58.1 58.1 1281-1282 bp 1.0 79.7
rps15-ycf1_p5 ACCATGCATTGATGGGTGTT CCTTATTGAGCCACTCCGAC 45.0 55.0 58.1 58.1 1278-1279 bp 1.0 79.7