Hotspot

psbT-psbN

LSC · 73955-74069 · 115 bp

Back to Myosurus

Pi 0.0783
MarkerSeek score 84.80
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
115
variable_sites
9
pi
0.0783
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 TTTTTCGAGAGCCGCCTAAG GGCGACCCTCTCAACAATTA 50.0 50.0 58.3 57.6 203-209 bp 1.0 78.1
psbT-psbN_p2 CGCCTAAGGTTCCGACTAAA GGCGACCCTCTCAACAATTA 50.0 50.0 57.3 57.6 191-197 bp 1.0 75.3
psbT-psbN_p3 CGCCTAAGGTTCCGACTAAAA GGCGACCCTCTCAACAATTA 47.6 50.0 58.0 57.6 191-197 bp 1.0 72.2
psbT-psbN_p4 TTTTTCGAGAGCCGCCTAAG AAGTTTTACCGGGTATGCCT 50.0 45.0 58.3 56.8 238-244 bp 1.0 71.3
psbT-psbN_p5 TTTTTCGAGAGCCGCCTAAG AGTTTTACCGGGTATGCCTT 50.0 45.0 58.3 56.8 237-243 bp 1.0 71.3