Hotspot

trnT-GGU-psbD

LSC · 29721-31131 · 1411 bp

Back to Myosurus

Pi 0.00425
MarkerSeek score 61.20
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1411
variable_sites
6
pi
0.00425
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GCCCTTTTAACTCAGCGGTA AACCGACAAAAACGAAACGG 50.0 45.0 57.9 57.8 1580 bp 1.0 78.1
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA TCTCCGTAACCAGTCATCCA 50.0 50.0 57.9 57.8 1558 bp 1.0 77.7
trnT-GGU-psbD_p3 GCCCTTTTAACTCAGCGGTA GAAGAGCAATAGACCCGACC 50.0 55.0 57.9 57.8 1600 bp 1.0 77.7
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA GGAAGAGCAATAGACCCGAC 50.0 55.0 57.9 57.8 1601 bp 1.0 77.7
trnT-GGU-psbD_p5 GCCCTTTTAACTCAGCGGTA CCGACAAAAACGAAACGGTC 50.0 50.0 57.9 58.3 1578 bp 1.0 77.3