Hotspot

petN-psbM

LSC · 26459-27434 · 976 bp

Back to Myosurus

Pi 0.00512

MarkerSeek score 61.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 976
variable_sites: 5
pi: 0.00512
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TGGGCTGCTCTAATGGTAGT	TCATTGCTACTGCCCTGTTC	50.0	50.0	58.1	57.9	1128 bp	1.0	78.5
petN-psbM_p2	TGGGCTGCTCTAATGGTAGT	TGCTACTGCCCTGTTCATTC	50.0	50.0	58.1	57.9	1124 bp	1.0	78.5
petN-psbM_p3	TGGGCTGCTCTAATGGTAGT	CATTGCTACTGCCCTGTTCA	50.0	50.0	58.1	58.2	1127 bp	1.0	78.0
petN-psbM_p4	AGTAAGTCTTGCTTGGGCTG	TCATTGCTACTGCCCTGTTC	50.0	50.0	57.8	57.9	1141 bp	1.0	77.9
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCCCTGTTCATTC	50.0	50.0	57.8	57.9	1137 bp	1.0	77.9