Hotspot

atpH-atpI

LSC · 12108-13035 · 928 bp

Back to Myosurus

Pi 0.00647

MarkerSeek score 62.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 928
variable_sites: 6
pi: 0.00647
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.0	57.9	990-995 bp	1.0	79.7
atpH-atpI_p2	CGCAATACCTTCTACGGCTT	ATAGGGGAATCCATGGAGGG	50.0	55.0	58.1	57.9	1078-1083 bp	1.0	79.4
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	57.9	57.9	1006-1011 bp	1.0	78.8
atpH-atpI_p4	GCAGCAGTACCTTGACCAAT	ATAGGGGAATCCATGGAGGG	50.0	55.0	58.2	57.9	1053-1058 bp	1.0	78.5
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.2	57.9	991-996 bp	1.0	78.5