Hotspot

psbK-psbI

LSC · 6219-6625 · 407 bp

Back to Myosurus

Pi 0.0147

MarkerSeek score 63.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 407
variable_sites: 6
pi: 0.0147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTTTATTCCTCACGCCCAGG	50.0	50.0	57.9	57.9	550 bp	1.0	79.4
psbK-psbI_p2	CAAATTGCCCGAAGCTTACG	TTTTATTCCTCACGCCCAGG	50.0	50.0	58.1	57.9	635 bp	1.0	78.9
psbK-psbI_p3	CCAAATTGCCCGAAGCTTAC	TTTTATTCCTCACGCCCAGG	50.0	50.0	57.7	57.9	636 bp	1.0	77.5
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	TTTTATTCCTCACGCCCAGG	45.0	50.0	58.3	57.9	547 bp	1.0	77.4
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	TTTTATTCCTCACGCCCAGG	45.0	50.0	58.3	57.9	548 bp	1.0	77.4